Restriction fragment length polymorphism analysis of the flaA gene of Campylobacter jejuni for subtyping human, animal and poultry isolates
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چکیده
منابع مشابه
Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene.
The Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, Calif.) utilizes capillary electrophoresis on a microchip device (LabChip 7500; Caliper Technologies, Mountain View, Calif.) that is capable of rapidly sizing small DNA fragments. To determine whether the system could replace conventional restriction fragment length polymorphism (RFLP) typing by agarose gel electrophoresis, we compa...
متن کاملRapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene.
A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified...
متن کاملCampylobacter jejuni and Campylobacter coli genotyping by high-resolution melting analysis of a flaA fragment.
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It w...
متن کاملgenetic characterization of campylobacter jejuni and c. coli isolated from broilers using flaa pcr-restriction fragment length polymorphism method in shiraz, southern iran
results all 90 campylobacter isolates confirmed by m-pcr were successfully typed using flaa-pcr-rflp. eleven different types were defined according to flaa-typing method and the rflp patterns were located at three separate clusters in rflp image analysis dendrogram. conclusions campylobacter jejuni isolates significantly showed more variety than c. coli isolates. a relatively low genetic divers...
متن کاملEvaluation of phenotypic and genotypic methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle.
Six methods for subtyping of Campylobacter jejuni were compared and evaluated with a collection of 90 isolates from poultry, cattle, and sporadic human clinical cases as well as from a waterborne outbreak. The applied methods were Penner heat-stable serotyping; automated ribotyping (RiboPrinting); random amplified polymorphic DNA typing (RAPD); pulsed-field gel electrophoresis (PFGE); restricti...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(99)00258-x